Abstract
To Explore Genes Related to the Prognosis of Colorectal Cancer
Department of Gastrointestinal Surgery, The Second Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan 421001, 1Department of Pre-Hospital First Aid, 2Department of General Surgery, Zhuzhou Central Hospital, Zhuzhou, Hunan 412000, China
Correspondence Address:
Chunyun Wang, Department of Gastrointestinal Surgery, The Second Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan 421001, China, E-mail: 892357467@qq.com
To explore the genes correlated with the prognosis in colorectal cancer is the main objective of the study. Identification of differential genes through series of GSE20970, GSE37182, GSE44861 and GSE64392 from gene expression Omnibus database and then the differential genes were analyzed by gene ontology and Kyoto encyclopedia of genes and genomes enrichment. Through protein-protein interaction networks, screening of key genes was done and then we analyzed the prognosis of colorectal cancer from the cancer genome Atlas database. We obtained the differential genes through limma screening in series data GSE20970, GSE37182, GSE44861 and GSE64392 from gene expression Omnibus database. Venn diagram was used to screen 206 intersecting differentially expressed genes. Gene ontology enrichment analysis terms used were cytosol, transcription, deoxyribonucleic acid-templated, nucleic acid-templated transcription, ribonucleic acid biosynthetic process, nucleobase-containing compound biosynthetic process, heterocycle biosynthetic process, aromatic compound biosynthetic process, regulation of nucleobase-containing compound metabolic process, regulation of ribonucleic acid metabolic process, organic cyclic compound biosynthetic process. Kyoto encyclopedia of genes and genomes pathway terms were interleukin-17 signaling pathway, bladder cancer, phospholipase D signaling pathway, human cytomegalovirus infection, aldosterone-regulated sodium reabsorption, Kaposi’s sarcoma-associated herpesvirus infection, forkhead box O signaling pathway, nuclear factor kappa B signaling pathway, hepatitis B. Protein-protein interaction network has shown 206 key genes. According to the top 50 of betweenness value, we analyzed the prognosis of colorectal cancer from the cancer genome Atlas database. We found Ki-ras2 Kirsten rat sarcoma viral oncogene homolog, C-X-C motif chemokine ligand 8, partner and localizer of breast cancer gene 2, peroxiredoxin 4 and translocase of outer mitochondrial membrane 20 are the key genes which impact prognosis in colorectal cancer. We predict that these genes can promote the survival time of colorectal cancer in future.
