Abstract
The Effect of Azalea on Oxidative Stress, Immune Factors and Vasodilator Factor in Hypertensive Rats
Cerebrovascular Disease Center, Gansu Provincial Hospital, Lanzhou,1Radiological Department, The Second People’s Hospital of Jiuquan City, Gansu 735000, P.R. China
Correspondence Address:
Erqing Chai, Cerebrovascular Disease Center, Gansu Provincial Hospital, Lanzhou, P.R. China, E-mail: biti4036@126.com
To analyze the effects of azalea on oxidative stress, immune factors and vasodilator factors in hypertensive rats. 24 male Specific-pathogen-free grade spontaneous hypertension rats were divided into model group (normal saline), azalea group (50 mg/kg/d azalea), verapamil group (50 mg/kg/d verapamil) and 8 normal control group (normal saline) and observed the changes of tail artery systolic pressure of rats for 2-18 w after administration, superoxide dismutase, malondialdehyde were detected after 18 w The levels of nuclear factor kappa B, Smooth muscle alpha-actin and Smooth muscle alpha actin were detected by reverse transcription–polymerase chain reaction and Western blot. The systolic pressure of the caudal artery in azalea group and verapamil group was significantly lower than that in the model group (p<0.05). The levels of Malondialdehyde and reactive oxygen species in azalea group and verapamil group were significantly lower than that in the model group and Superoxide dismutase level was significantly higher than that in the model group (p<0.05). The levels of interleukin-6 and tumor necrosis factor-alpha in azalea group and verapamil group were significantly lower than those in the model group (p<0.05). The levels of Angiotensin α and endothelin-1 in azalea group and verapamil group were significantly lower than that in the model group and no and nitric oxide synthase levels were significantly higher than those in the model group (p<0.05). The expression of nuclear factor kappa light chain enhancer of activated B cells messenger Ribonucleic acid and protein in azalea group and verapamil group was significantly lower than that in model group and Smooth muscle alpha-actin messenger Ribonucleic acid and protein were significantly higher than that in model group (p<0.05). Azalea can significantly reduce the systolic pressure of hypertensive rats and protect the aortic injury. It may be related to the reduction of oxidative stress, regulation of immune factors and diastolic vasodilator factors, and regulation of nuclear factor kappa light chain enhancer of activated B cells, Smooth muscle alpha-actin expressions.
