Abstract
Study on the Mechanism of Long-Non-Coding RNA MALAT1 Affecting Apoptosis and Invasion of Meningioma Cells through Phosphoinositide 3-Kinase/Protein Kinase B Signal Pathway
Department of Neurosurgery, The Second Affiliated Hospital of Xingtai Medical College, Xingtai 054000, 1Department of Critical Care Medicine, Fourth Hospital of Hebei Medical University, Shijiazhuang 050000, 2Department of Drug Dispensing, The Second Affiliated Hospital of Xingtai Medical College, Xingtai, Hebei Province 054000, China
Correspondence Address:
Lixia Zhang, Department of Drug Dispensing, The Second Affiliated Hospital of Xingtai Medical College, Xingtai, Hebei Province 054000, China, E-mail: zjfhao001@163.com
To study the mechanism of long-non-coding RNA metastasis associated lung adenocarcinoma transcript 1 affecting apoptosis and invasion of meningioma cells through phosphoinositide 3-kinase/protein kinase B signal pathway. Meningioma CH157 cell lines were cultured and divided into control (group A), long-non-coding RNA metastasis associated lung adenocarcinoma transcript 1 knockout (group B) and low expression of long non-coding RNA metastasis associated lung adenocarcinoma transcript 1+phosphoinositide 3-kinase activator (740Y-P) (group C). The apoptosis rate of meningioma cells CH157 was detected by flow cytometry and the invasion of meningioma cells were observed by Transwell test. The expression of messenger ribonucleic acid and protein were detected by reverse transcription-quantitative polymerase chain reaction and Western blot method. The apoptosis rate of meningioma cells in group B was increased than group A, while that in group C was lower than group B. The invasion number of meningioma cells in group B was increased than group A, and that in group C was reduced than group B. The expression of B-cell lymphoma 2 was decreased and the B-cell lymphoma 2-associated protein X was increased in group B than group A, and the B-cell lymphoma 2 and B-cell lymphoma 2-associated protein X in group C was raised than group B. The long-non-coding RNA metastasis associated lung adenocarcinoma transcript 1 in group B was reduced than group A. The protein p-phosphoinositide 3-kinase and p-protein kinase B in group B were decreased than group A, while these in group C were raised than group B. Long-non-coding RNA metastasis associated lung adenocarcinoma transcript 1 knockout promotes apoptosis and inhibits cell invasion by activating phosphoinositide 3-kinase/ protein kinase B signaling pathway. The activation of phosphoinositide 3-kinase/protein kinase B signal pathway can partially reverse the inhibitory effect of low expression of long-non-coding RNA metastasis associated lung adenocarcinoma transcript 1 on apoptosis and invasion of meningioma cells.
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