Abstract
Study on Serum MicroRNA-30a Expression and Cardioprotective Mechanism of Mongolian Medicine, Zadi-5 in Rats with Ischemia-Reperfusion Injury
Department of Medicine, Inner Mongolia Medical University, Hohhot, Inner Mongolia 010107, 1Department of Pharmacy, Dalian Medical University, Dalian, Liaoning Province 116044, China
Correspondence Address:
Hu Ri Le Ba Gen, Department of Medicine, Inner Mongolia Medical University, Hohhot, Inner Mongolia 010107, China, E-mail: hurile1997@163.com
Aim of this study was to study serum microribonucleic acid-30a expression and the cardioprotective mechanism of Mongolian medicine, Zadi-5 in myocardial ischemia-reperfusion injury model rats. 84 Sprague-Dawley male rats were selected and were divided into 7 groups, namely blank, model, sham operation, Chinese medicine, as well as Mongolian medicine high, medium and low dose groups, each with 12 rats. Among them, the Chinese medicine group was intervened by compound Danshen dripping pills while the Mongolian medicine (Zadi- 5) of high (1.6 g/kg), medium (0.8 g/kg) and low dose (0.4 g/kg) groups were given and the blank group was given an equal volume of distilled water of 1.0 ml/100 g. Myocardial ischemia-reperfusion injury rat model was established and the model was evaluated by electrocardiogram. In addition, serum and cardiac tissues of each group were collected to measure serum aspartate aminotransferase, lactate dehydrogenase and lactate dehydrogenase-1 contents; infarct area and cardiomyocyte apoptosis rate of rats were detected. Furthermore, the morphological changes of myocardial tissue, B cell lymphoma-2 and B cell lymphoma-2- associated protein X expression and microribonucleic acid-30a levels in myocardial tissue were determined. The experimental results showed successful construction of the MIRI rat model. Meanwhile, the arrangement of myocardial cells in the model group was disordered, accompanied by cell rupture, necrosis, inflammatory cell infiltration and myocardial fiber rupture; while the Chinese medicine and Mongolian medicine groups had reversed histological changes to varying degrees. The model group showed markedly reduced B cell lymphoma-2 protein expression and microribonucleic acid-30a levels in myocardial tissue than in blank and sham operation groups; however, B cell lymphoma-2 protein expression and miR-30a levels showed increasing trend of Chinese medicine as well as high and medium doses of Mongolian medicine. Zadi-5 may exert a cardioprotective effect by up-regulating serum microribonucleic acid-30a expression in ischemia-reperfusion injury rats.
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