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Abstract
Spectrophotometric method for estimation of rabeprazole
Author(s): PM Patel, HJ Desai, RC Patel, NM Patel
B. M. Shah College of Pharmaceutical Education & Research, Modasa - 383 315, India
Correspondence Address:
P M Patel B. M. Shah College of Pharmaceutical Education & Research, Modasa - 383 315 India E-mail: piyushpharma17@rediffmail.com
B. M. Shah College of Pharmaceutical Education & Research, Modasa - 383 315, India
Correspondence Address:
P M Patel B. M. Shah College of Pharmaceutical Education & Research, Modasa - 383 315 India E-mail: piyushpharma17@rediffmail.com
A simple new spectrophotometric method has been developed for determination of rabeprazole in pharmaceutical bulk dosage form. The method was based on the formation of ion-pair complexes of the drug with four dyes, viz. bromo thymol blue, bromocresol green, bromophenol blue and bromocresol purple in acidic buffer solutions followed by their extraction in chloroform. The absorbance of the organic layer was measured at its respective wavelength of maximum absorbance against the corresponding reagent blank. The method has been statistically evaluated and was found to be precise and accurate. Phosphate buffer of pH 2 and bromocresol green dye gave maximum absorbance of rabeprazole at 454 nm.
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