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Abstract
Spectrophotometric method for analysis of metformin hydrochloride
Author(s): G Mubeen, Khalikha Noor
Al-Ameen College of Pharmacy, Hosur Road, Bangalore-560 027, India
Correspondence Address:
G Mubeen Al-Ameen College of Pharmacy, Hosur Road, Bangalore-560 027 India mubeenghani@hotmail.com
Al-Ameen College of Pharmacy, Hosur Road, Bangalore-560 027, India
Correspondence Address:
G Mubeen Al-Ameen College of Pharmacy, Hosur Road, Bangalore-560 027 India mubeenghani@hotmail.com
A simple and sensitive spectrophotometric method has been developed and validated for the estimation of metformin hydrochloride in bulk and in tablet formulation. The primary amino group of metformin hydrochloride reacts with ninhydrin in alkaline medium to form a violet colour chromogen, which is determined spectrophotometrically at 570 nm. It obeyed Beer's law in the range of 8-18 µg/ml. Percentage recovery of the drug for the proposed method ranged from 97-100% indicating no interference of the tablet excipients. The proposed method was found to be accurate and precise for routine estimation of metformin hydrochloride in bulk and from tablet dosage forms.
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