Abstract

Two new specific, selective, simple, rapid and inexpensive spectofluorophotometric methods have been developed for the determination of rofecoxib and mosapride citrate in their individual dosage forms. Spectra of rofecoxib in 0.1 N sulphuric acid and mosapride in methanol showed excitation wavelength to be 282 nm and 331 nm while emission wavelength to be 406 nm and 360 nm respectively. Method for rofecoxib was found to be linear over an analytical range of 10-50 ng/ml with correlation coefficient of 0.9990. Limit of detection and limit of quantitation were found to be 1.71 ng/ml and 5.69 ng/ml, respectively for the same method. Method for mosapride was found to be linear over an analytical range of 2-10 ng/ml with correlation coefficient of 0.9996. Limit of detection and limit of quantitation were found to be 0.32 ng/ml and 1.05 ng/ml, respectively for the same method. Both the methods were validated and found to be suitable for estimation of rofecoxib and mosapride from their individual pharmaceutical formulations. Satisfactory recovery from the spiked samples of standard drugs suggests no interference of any excipients present in the formulations.