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Abstract
Simultaneous spectrophotometric estimation of tenofovir disoproxil fumarate and lamivudine in three component tablet formulation containing efavirenz
Author(s): R Sharma, K Mehta
School of Pharmacy, Devi Ahilya University, Takshshila Campus, Khandwa Road, Indore-452 001, India
Correspondence Address:
E-mail: rbsm73@yahoo.co.in
School of Pharmacy, Devi Ahilya University, Takshshila Campus, Khandwa Road, Indore-452 001, India
Correspondence Address:
E-mail: rbsm73@yahoo.co.in
Three UV spectrophotometric methods have been developed, simultaneous equation method, multicomponent analysis (II) and derivative spectroscopy method (III). The absorption maxima of the drugs were found to be 247, 259 and 272 nm, respectively for efavirenz, tenofovir disoproxil fumarate and lamivudine in methanol:water (50:50) solvent system. Efavirenz, tenofovir disoproxil fumarate and lamivudine obeyed Beer's law in the concentration range of 10-60, 5-30 and 5-30 μg/ml, respectively. Results of analysis for all the three methods were analyzed and validated for various parameters according to ICH guidelines.
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