Abstract

The study was designed to develop a simple, precise, rapid and reproducible simultaneous method for fosamprenavir calcium and nevirapine in dendrimer-carbon nanotube conjugates. Reverse phase column C-18 was used for the development and validation of the high-performance liquid chromatography method. The method was run on isocratic mode with methanol and water (80:20) with 1 ml/min flow rate and the detection was carried out at 258 nm. Fosamprenavir calcium was shown to have a retention time of 4.255 min whereas nevirapine had a retention time of 6.337 min. The method was validated as per International Council for Harmonisation M10 guidelines. The coefficient of correlation (R²) was found to be 0.9905 for fosamprenavir calcium and 0.9896 for nevirapine. The approach was accurate and precise based on drug recovery (greater than 95 %) and relative standard deviation (less than 2 % among duplicate experiments). Lower, medium and higher quantified concentration of fosamprenavir calcium were found to be 5.98 µg/ml, 7.18 µg/ml and 8.38 µg/ml, respectively and for nevirapine the lower, medium and higher quantified concentration were found to be 6.54 µg/ml, 7.74 µg/ml and higher quantified concentration 8.94 µg/ml, respectively. Drug loading was found to be 47.59 % for fosamprenavir calcium and 69.65 % for nevirapine when both drugs are loaded together in dendrimer-carbon nanotube conjugates. From the study, it can be concluded that a robust method was developed that qualified different validation parameters and can be used for the determination of fosamprenavir calcium and nevirapine in dendrimer-carbon nanotube conjugates.