Abstract
Silencing APRIL Gene by siRNA Leads to Inhibition of Proliferation and Cell Cycle Arrest in Gastric Cancer Cell Line MGC-803 and SGC-7901
Department of Digestive Endoscopy, The Second Hospital of Jilin University, Jilin, China
Correspondence Address:
Department of Digestive Endoscopy, The Second Hospital of Jilin University, Jilin, China, E-mail: ndkui@sina-com.tk
The aim of this study was to explore the effects of silencing a proliferation-inducing ligand on the growth and cell cycle of human gastric cancer cells. MGC-803 and SGC-7901 cells were infected with lenti-a proliferation-inducing ligand si and mRNA expression was analyzed by real-time polymerase chain reaction. Cell viability, colony formation and cell cycle stages were detected by MTT assay, colony forming assay and Cellomics ArrayScan, respectively. Results from real-time polymerase chain reaction indicated that lenti-a proliferation-inducing ligand-si greatly reduced the proliferation-inducing ligand mRNA expression in both cell lines. Cell viability and colony formation ability were significantly hampered. Moreover, cell cycle was arrested in both cell lines, elucidating the mechanism underlying the inhibitory effects of siRNA on cell proliferation. The present study indicated that lentivirus-mediated gene delivery might be a promising strategy in the treatment of gastric cancer.
