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Abstract
Sensitive fluorometric method for the determination of rutin in combined tablet dosage form
Author(s): SF Liu
School of Public Health, Shandong University, Jinan, 250012, China
Correspondence Address:
S F Liu School of Public Health, Shandong University, Jinan, 250012 China E‑mail: liushufang@sdu.edu.cn
School of Public Health, Shandong University, Jinan, 250012, China
Correspondence Address:
S F Liu School of Public Health, Shandong University, Jinan, 250012 China E‑mail: liushufang@sdu.edu.cn
A sensitive method based on the fluorescence enhancement was developed for the determination of rutin. It was found that the rutin could form a fluorescent complex with ytterium (III), and the fluorescence intensity of which could be enhanced by sodium dodecyl benzene sulfonate. Under the optimum conditions, the enhanced fluorescence intensity was in proportion to the concentration of rutin in the range of 12-1200 ng/ml and the detection limit (S/N=3) was 1.3 ng/ml. This method possessed the properties of simplicity, fast assay process and high sensitivity, and has been satisfactorily applied for the analysis of actual sample. The possible interaction mechanism of this system was also discussed in this manuscript.
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