Abstract

Rutin-induced changes in apoptosis and reduced glutathione levels was studied in murine ascites Dalton’s lymphoma cells under different treatment conditions followed by the binding affinity of rutin and cis-diamminedichloroplatinum (II) with two antiapoptotic proteins and two glutathione-related enzymes was examined through molecular docking analysis. Rutin, as well as cis-diamminedichloroplatinum (II) induced apoptosis and a decrease in reduced glutathione level in Dalton’s lymphoma cells. The molecular interactions of rutin, cis-diamminedichloroplatinum (II) and a known inhibitor of the protein/enzyme showed that the binding free energies (ΔG) with antiapoptotic proteins, Bcl-xL and c-FLIP, and glutathione-related enzymes such as glutathione S-transferase and glutathione reductase were closer and comparable, which might suggest inhibitory potential of rutin on these proteins/enzymes and could play a role in its anticancer activity.