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Abstract
RPLC determination of tinidazole and diloxanide furoate in tablets
Author(s): P.N.S Pai, GK Rao, B Srinivas, S Puranik
Department of Quality Assurance, Al-Ameen College of Pharmacy, Bangalore-560027, India
Correspondence Address:
P.N.S Pai Department of Quality Assurance, Al-Ameen College of Pharmacy, Bangalore-560027 India E-mail: pnsanjaypai@rediffmail.com
Department of Quality Assurance, Al-Ameen College of Pharmacy, Bangalore-560027, India
Correspondence Address:
P.N.S Pai Department of Quality Assurance, Al-Ameen College of Pharmacy, Bangalore-560027 India E-mail: pnsanjaypai@rediffmail.com
A simple reverse phase liquid chromatographic method has been developed and subsequently validated for simultaneous determination of tinidazole and diloxanide furoate. The separation was carried out using a mobile phase consisting of acetonitrile, methanol and 0.2 M potassium dihydrogen phosphate (pH 5) in the ratio 2:3:2.The column used was SS Wakosil-II C-18 with a flow rate of 1 ml/min and UV detection at 282 nm. The described method was linear over a concentration range of 10-70 mg/ml and 10-90 mg/ml for the assay of diloxanide furoate and tinidazole, respectively. The mean recovery was found to be 100-101% for tinidazole and 97-103% for diloxanide furoate when determined at three different levels.
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