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Abstract
RP-HPLC method for the estimation of dutasteride in tablet dosage form
Author(s): Dipti B Patel, NJ Patel, SK Patel, AM Prajapati, SA Patel
S. K. Patel College of Pharmaceutical Education & Research, Department of Pharmaceutical Chemistry, Ganpat University, Kherva-382 711,
Correspondence Address:
Dipti B Patel S. K. Patel College of Pharmaceutical Education & Research, Department of Pharmaceutical Chemistry, Ganpat University, Kherva-382 711 E-mail: diptibpatel_24980@yahoo.co.in
S. K. Patel College of Pharmaceutical Education & Research, Department of Pharmaceutical Chemistry, Ganpat University, Kherva-382 711,
Correspondence Address:
Dipti B Patel S. K. Patel College of Pharmaceutical Education & Research, Department of Pharmaceutical Chemistry, Ganpat University, Kherva-382 711 E-mail: diptibpatel_24980@yahoo.co.in
A simple, sensitive and precise RP-HPLC method was developed for the determination of dutasteride in tablet dosage form. The RP-HPLC separation was achieved on phenomenex C 18 column (250 mm, id 4.6 mm, 5 µm) using mobile phase methanol:water (90:10 v/v) at a flow rate of 1 ml/min at an ambient temperature. Quantification was achieved with photodiode array detection at 235 nm over the concentration range 1-12 µg/ml. The method was validated statistically and was applied successfully for the determination of dutasteride in tablets.
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