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Abstract
RP-HPLC estimation of Raloxifene hydrochloride in tablets
Author(s): DC Pavithra, Lakshmi Sivasubramanian
Department of Chemistry, Pharmaceutical Chemistry Unit, Vellore Institute of Technology, Deemed University, Vellore-632 014, India
Correspondence Address:
Lakshmi Sivasubramanian Department of Chemistry, Pharmaceutical Chemistry Unit, Vellore Institute of Technology, Deemed University, Vellore-632 014 India E-mail: lakshmiss@hotmail.com
Department of Chemistry, Pharmaceutical Chemistry Unit, Vellore Institute of Technology, Deemed University, Vellore-632 014, India
Correspondence Address:
Lakshmi Sivasubramanian Department of Chemistry, Pharmaceutical Chemistry Unit, Vellore Institute of Technology, Deemed University, Vellore-632 014 India E-mail: lakshmiss@hotmail.com
A simple, efficient, and reproducible method for the determination of raloxifene hydrochloride in tablets has been developed using reverse phase high performance liquid chromatographic method. The elution was done using a mobile phase consisting of methanol and water (50:50 v/v) on Water's Symmetry C 18 4.6x150 mm analytical column with flow rate of 1 ml/min with detection at 230 nm. An external standard calibration method was employed for quantitation. The elution time was 4.1 min. The linearity range was 10-60 mg/ml for raloxifene hydrochloride.
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