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Abstract
Quantitative Determination Of Bacoside By HPLC
Author(s): R Pal, A. K Dwivedi, S Singh, D. K Kulshreshtha
High pressure liquid chromatographic method for quantitative determination of Bacoside-A3 the main active constituent of the saponin fraction having facilitatory effect on learning is described. A C18 column was used for the separation with acetonitri1e: water (40:60) as mobile phase. The lowest quantitation limit was 26 mg/ml. The intra-day relative standard deviations and deviation from actual concentration values are less 6% and ± 8% respectively, which showed good reproducibility and accuracy of the method.
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