Abstract
Pterostilbene Enhances Anticancer Effects of L-asparaginase in Lymphoblastic Leukemia Cell Line
Immunology Department, Shahrekord University of Medical Sciences, 1Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, 2School of Medicine, Tehran University of Medical Sciences, Tehran, 3Pathology and Hematology Department, Shahrekord University of Medical Sciences, Shahrekord, 4Medical Plant Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
Correspondence Address:
Pathology and Hematology Department, Shahrekord University of Medical Sciences, Shahrekord, Medical Plant Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran, E-mail: Bat238@yahoo.com
Antiproliferative and apoptotic effects of pterostilbene were examined in combination with L-asparaginase in Jurkat cell line. Jurkat cells were incubated with different concentrations of pterostilbene alone or in combination with L-asparaginase for 24, 48 and 72 h. Cell viability was evaluated using 3-(4,5-dimethylthiazol-2-yl)-5-3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2htetrazolium assay. Induction of apoptosis was measured by annexin-V fluorescein isothiocyanate and the level of active caspase 3 positive cells by intracellular staining and flowcytometry. Decline of cell viability to 50 % was observed at 67.78±3.88, 60.97±3.36 and 52.11±2.50 µM concentration after 24, 48 and 72 h incubation with pterostilbene, respectively. Pterostilbene at a concentration of 30, 50 and 70 µM in combination with 0.5 and 0.7 IU/ml L-asparaginase reduced relative cell growth to a significant level. The rate of apoptosis was significantly higher than control at 80 µM concentration of pterostilbene and a combination of 60 µM pterostilbene with 0.5 and 0.7 IU/ml L-asparaginase, but not with L-asparaginase alone. The level of caspase 3 positive cells was significantly higher than control at 80 µM concentration of pterostilbene. Pterostilbene increased antiproliferative and apoptotic effects of L-asparaginase in Jurkat cells. These results suggested that pterostilbene might be a potential anticancer agent in lymphoblastic leukaemia and potentiate the effect of L-asparaginase. Unravelling the mechanism of pterostilbene-induced cell apoptosis in this cell line could help in the development of a targeted therapy.
