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Abstract
Preparation and characterization of 125 I labeled bovine serum albumin
Author(s): KS Ashwitha Rai1, Jyothi1, RR Rasmi1, Jayula Sarnaik2, VB Kadwad2, KB Shenoy1, HM Somashekarappa1
1Centre for Application of Radioisotopes and Radiation Technology (CARRT), Mangalore University, Mangalagangothri 574 199, India 2Radiopharmaceuticals Production Programme, Board of Radiation and Isotope Technology (BRIT), Vashi Complex, Sector 20, Vashi, Navi Mumbai 400 705, India
Correspondence Address:
K B Shenoy Centre for Application of Radioisotopes and Radiation Technology (CARRT), Mangalore University, Mangalagangothri 574 199 India E-mail: kshenoyb@gmail.com
1Centre for Application of Radioisotopes and Radiation Technology (CARRT), Mangalore University, Mangalagangothri 574 199, India 2Radiopharmaceuticals Production Programme, Board of Radiation and Isotope Technology (BRIT), Vashi Complex, Sector 20, Vashi, Navi Mumbai 400 705, India
Correspondence Address:
K B Shenoy Centre for Application of Radioisotopes and Radiation Technology (CARRT), Mangalore University, Mangalagangothri 574 199 India E-mail: kshenoyb@gmail.com
Bovine serum albumin is a model protein, which has been conventionally used as protein standard and in many areas of biochemistry, pharmacology and medicine. Radioiodination procedure for bovine serum albumin employing chloramine-T as an oxidant with slight modification was evaluated critically to establish the optimal conditions for the preparation of radiolabeled tracer ( 125 I-BSA) with required specific activity without impairing the immune reactivity and biological activity. Optimized radioiodination procedure involving 10 µg of chloramine-T along with 20 µg of sodium metabisulphite with 60 seconds incubation at 2° yielded 125 I-BSA with high integrity.
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