Abstract

The goal of this research is to examine the pharmacokinetics of Zandopa® in rats after oral administration of Zandopa^® herbal formulation. Acclimatization of rats was done for 7 d before the study. The study consisted of 24 rats which had been separated equally into two groups i.e. vehicle control and Zandopa® control group with 12 animals in each group. The drug was administered via oral route in male Sprague Dawley rats. The vehicle control group rats were dosed with 1 % carboxymethyl cellulose and Zandopa^® control group with 775 mg/kg of Zandopa^® in 1 % carboxymethyl cellulose. Food was with-held for a further 1 h. The blood sample was taken between 0, 0.5, 1, 2, 4, 6, 8, 12 and 24 h time intervals after treatment with the respective drug. Plasma samples were prepared by centrifugation and concentration was measured by high performance liquid chromatography. Urine was collected at a time interval of 0-1, 1-4, 4-8, 8-24, 24-48 h after treatment with the respective drug, and kinetic parameters were measured. Then animals were sacrificed using isoflurane overdose and the liver was isolated and stored for metabolism study. The Cytochrome P-450 concentration and Cytochrome P-450 content was found to be 0.0012 mM and 1.2 nmol/mg/protein compared with the vehicle control group. The cumulative amount excreted was found to be 64.66 mg. The study of the pharmacokinetics of Zandopa® in rats was shown to be significant as compared to the vehicle control group.