Abstract

Sepsis is a common infectious disease, and myocardial injury associated with sepsis is a major contributor to increased mortality. This work aimed to demonstrate the mechanisms by which a Shenfu injection solution regulates the nuclear factor erythroid 2-related factor 2, heme oxygenase-1, glutathione peroxidase 4, and ferroptosis pathway to alleviate sepsis-induced myocardial injury. Seventy-five C57BL/6J mice were selected and rolled into the following groups; control group, sham operation group (S1), positive control group (Y1), model group (M0), and Shenfu injection solution group (M1). After model establishment, each group received the corresponding treatment. Glutathione peroxidase 4, acetyl coenzyme A synthetize long chain family member 4, and polyunsaturated fatty acid coenzyme A levels were observed in the serum and myocardial tissues of the mice in each group. Experimental observations revealed that the glutathione peroxidase 4 expression was notably downregulated in the M0 group (p<0.05), while acetyl coenzyme A synthetize long chain family member 4 and polyunsaturated fatty acid coenzyme A was greatly upregulated, accompanied by a noticeable increase in myocardial injury (p<0.05). In contrast, glutathione peroxidase 4 was markedly upregulated (p<0.05), while acetyl coenzyme A synthetize long chain family member 4 and polyunsaturated fatty acid coenzyme A level was drastically downregulated (p<0.05) in the M1 group, leading to a remarkable reduction in myocardial injury. Shenfu injection solution can alleviate sepsis-induced myocardial injury by regulating the nuclear factor erythroid 2-related factor 2/heme oxygenase-1/glutathione peroxidase 4 ferroptosis pathway, downregulating acetyl coenzyme A synthetize long chain family member 4 and polyunsaturated fatty acid coenzyme A level, and upregulating glutathione peroxidase 4 level.