Abstract

Shenxiong-Xinmaikang decoction has good clinical efficacy in the treatment of stable angina pectoris, but its active compounds and mechanism of action remain unclear. The aim of the study was to explore the potential mechanisms of Shenxiong-Xinmaikang decoction on stable angina pectoris. Shenxiong-Xinmaikang decoction components were obtained from the traditional Chinese medicine systems pharmacology, encyclopedia of traditional Chinese medicine, and SymMap databases, and potential targets were obtained from the Swiss TargetPrediction database. Stable angina pectoris targets were predicted using GeneCards, Online Mendelian Inheritance in Man, and DrugBank databases. The overlapping targets and their network were obtained; subsequently, the 'drugs-active ingredients-targets-disease' network, protein-protein interaction networks, and hub genes network were constructed using Cytoscape. Characterization of biological functions and pathways of potential targets through gene oncology and kyoto encyclopedia of genes and genomes enrichment. Molecular docking was performed using AutoDock 4.2.6 and PyMOL 2.4.0 software. 97 compounds, 829 corresponding targets, 728 stable angina pectoris-related targets, and 183 overlapping targets were identified. The network analysis revealed 9 key components and 14 key targets. Simultaneously, molecular docking analysis confirmed that these compounds had a good affinity with the targets. Gene oncology enrichment analysis showed that most of the common targets were mainly involved in the biological process of mitogen-activated protein kinase cascade regulation and cell response to organic nitrogen compounds, involving the membrane raft, the membrane microdomain and the caveola, and correlated with molecular functions such as related kinase activity and kinase binding. Kyoto encyclopedia of genes and genomes enrichment analysis showed that the overlapping targets were primarily involved in phosphatidylinositol 3-kinase/protein kinase B signaling and mitogen-activated protein kinase signaling. Our study mainly found that Shenxiong-Xinmaikang decoction might treat stable angina pectoris from the perspective of inflammation and lipid regulation, providing a theoretical basis for drug development.