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Abstract
Isolation and catalytic competence of different animal liver microsomal fractions prepared by calcium-aggregation method
Author(s): Pradnya S Walawalkar, Pooja S Serai, KR Iyer
Department of Pharmaceutical Chemistry, Bombay College of Pharmacy, Kalina, Mumbai-400 098, India
Correspondence Address:
K R Iyer Department of Pharmaceutical Chemistry, Bombay College of Pharmacy, Kalina, Mumbai-400 098 India E-mail: krishnaiye@gmail.com
Department of Pharmaceutical Chemistry, Bombay College of Pharmacy, Kalina, Mumbai-400 098, India
Correspondence Address:
K R Iyer Department of Pharmaceutical Chemistry, Bombay College of Pharmacy, Kalina, Mumbai-400 098 India E-mail: krishnaiye@gmail.com
Rat, mouse, rabbit, and guinea pig liver microsomes were prepared, using the calcium-aggregation method. The mean specific spectral cytochrome P450 content obtained for rat, mouse, rabbit, and guinea pig liver microsomes were 0.547, 0.394, 0.677, and 0.378 nmol cytochrome P450/mg protein, respectively. All the microsomal samples showed the ability to biotransform p-nitrophenol to p-nitrocatechol. The mean rate of formation of p-nitrocatechol by rat, mouse, rabbit, and guinea pig liver microsomes was 0.272, 0.247, 0.497 and 0.424 nmole/min/nmole cytochrome 450, respectively. This method circumvents the need of an ultracentrifuge for isolation of microsomes, by the conventional differential centrifugation method.
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