Abstract
Investigating the Stability of Enterovirus 71 in Antiseptics and Environmental Variables
Department of Preventive Drug Research, Guangdong Provincial Institute of Biological Products and Materia Medica, Guangzhou 510440, 1Guangdong Pharmaceutical University, Guangzhou 510006, 2Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430, China
Correspondence Address:
Lei Zhang, Department of Preventive Drug Research, Guangdong Provincial Institute of Biological Products and Materia Medica, Guangzhou 510440, China, E-mail: swzl100@sina.com
The model virus is crucial for evaluating virucidal activity of disinfectants. However, the utilization of poliovirus is only temporary because of the global polio eradication program. Enterovirus 71 has the advantages of high virus titer, convenient treatment and little harm, and can be used as a potential model virus for evaluating virus inactivation activity. To investigate resistance of enterovirus 71 to environmental (dry surfaces and hard water) and 10 hand disinfectants, compared with poliovirus-I virus. On dry surface, two viruses had shown reduction in activity with the increment of treating time and the activity of <4 log10TCID50 (log) at 4 h-treated time. However, neither poliovirus-I or enterovirus 71 in virus activity had maintained >4 log in hard water after treatment for 14 d. Six of 10 disinfectants reach the 4-log reduction requirement. Enterovirus 71 compared with poliovirus-I, exhibited the similar resistance to dry surface, hard water and disinfectants. Enterovirus 71 can be considered a suitable and important alternative model virus in the replacement of poliovirus-I to support the claims of virucidal activity. The model virus is crucial to evaluate the virucidal activity of disinfectants. However, the use of poliovirus is only temporary due to the global polio eradication program. Enterovirus 71 has the advantages of high viral titer, convenient treatment and little damage, and can be used as a potential model virus to evaluate virus inactivation activity. To investigate the resistance of enterovirus 71 to the environment (dry surfaces and hard water) and 10 hand sanitizers, compared to the poliovirus-I virus. On dry surfaces, two viruses showed a reduction in activity with increasing treatment time and an activity of <4 log10TCID50 (log) at 4 h of treatment time. However, neither poliovirus-I nor enterovirus 71 virus activity remained >4 log in hard water after treatment for 14 d. 6 out of 10 disinfectants meet the 4 log reduction requirement. Enterovirus 71, compared to poliovirus-I, showed similar resistance to dry surfaces, hard water and disinfectants. Enterovirus 71 may be considered a suitable and important alternative model virus replacing poliovirus-I to support claims of virucidal activity.
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