Abstract

To investigate the effect of etomidate on the proliferation, migration and invasion of glioma cells and its possible mechanism is the main objective. Glioma cells SW1088 were cultured in vitro and interfered with different doses of etomidate. Cell proliferation was detected by cell counting kit-8 method and cell migration and invasion were detected by Transwell. Long chain non-coding RNA homeobox A11 antisense RNA over expression vector or microRNA-515-5p mimetic or co-transfecting long chain non-coding RNA homeobox A11 antisense RNA over expression vector and microRNA-515-5p mimetic was transfected to SW1088 cells. After etomidate treatment of glioma SW1088 cells, the expression of microRNA-515-5p increased, but A value of the cells, the number of migration and invasion, the protein expression levels of proliferating cell nuclear antigen, matrix metalloproteinase-2, matrix metalloproteinase-9 and long chain non-coding RNA homeobox A11 antisense RNA decreased (p<0.05), while they increased after 400 μmol/l etomidate interfered with SW1088 cells of over expressing long chain non-coding RNA homeobox A11 antisense RNA (p<0.05). Over expression of microRNA-515-5p reduced the A value of SW1088 cells, the number of migration and invasion and the expression levels of proliferating cell nuclear antigen, matrix metalloproteinase-2 and matrix metalloproteinase-9 proteins. Also, it could reverse the over expression of long chain non-coding RNA homeobox A11 antisense RNA on the proliferation, migration and invasion of SW1088 cells treated with etomidate. Etomidate may inhibit the proliferation, migration and invasion of glioma cells by regulating the long chain non-coding RNA homeobox A11 antisense RNA/microRNA-515- 5p axis.