Abstract
In vitro evaluation of Terminalia arjuna on calcium phosphate and calcium oxalate crystallization
1 Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Waknaghat, Solan-173 215, India 2 Department of Biochemistry, Panjab University, Chandigarh-160 014, India
Correspondence Address:
C Tandon Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Waknaghat, Solan-173 215 India E-mail: adaobiezike@yahoo.ca
Urinary stones are one of the oldest and the most common afflictions in humans. This disease has tormented humans since the earliest records of civilization. Ten percent of men and 3 % of women have a stone during their adult lives. Calcium containing stones are the most common comprising about 75 % of all urinary calculi, which may be in the form of pure calcium oxalate (50 %) or calcium phosphate (5 %) or a mixture of both (45 %). A number of plants have been mentioned in the Indian ayurvedic system, which plays a vital role in the inhibition of kidney stones. In the present study, the inhibitory potency of crude extracts or fractions of successive solvent extractions of Terminalia arjuna bark was evaluated on various stages of formation of calcium phosphate and on the growth of calcium oxalate monohydrate crystals in vitro. Results obtained indicated that Terminalia arjuna bark has the potential to inhibit the formation of both calcium phosphate and calcium oxalate crystals in vitro. Butanol fraction of Terminalia arjuna extract was the most effective in inhibiting formation of calcium phosphate and calcium oxalate crystals in vitro.
