Abstract
Impact of Different Culture Media on Proliferation and Dedifferentiation of Fatal Chondrocytes
Department of Obstetrics and Gynecology, Pudong New Area People’s Hospital, Shanghai 201299, 1Department of Obstetrics and Gynecology, Taizhou Second People’s Hospital of Jiangsu Province, Taizhou 225511, 2Department of Obstetrics and Gynecology, The First People’s Hospital of Nantong, Nantong 226000, 3Department of Obstetrics and Gynecology, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu 213001, 4Shanghai Woowoo Stem Cell Technology Co., Ltd., Shanghai 200042, 5Anhui Medical University, Anhui Province 230022, ChinaSuzhou, Jiangsu Province 215000, China
Correspondence Address:
Chunyan Xue, Department of Obstetrics and Gynecology, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu 213001, China, E-mail: 15895081921@139.com
Chondrocytes play a crucial role in modern tissue engineering; however, their in vitro cultivation often encounters challenges associated with dedifferentiation, posing a significant obstacle in the extracorporeal cultivation of these cells. The extracellular environment in vitro profoundly influences cellular states, encompassing cell morphology, proliferation, and gene expression. This study aimed to investigate the impact of different basal culture media and supplement factors on the proliferation and gene expression of fetal chondrocytes in vitro. The goal was to identify an optimal complete culture medium that retarded the dedifferentiation process during the extracorporeal cultivation of fetal chondrocytes. Chondrocytes were isolated and extracted from fetal cartilage tissue and cultured in vitro, and the morphological changes of chondrocytes were observed. The effects of various basal culture media and supplement factors on chondrocyte proliferation and gene expression were assessed using cell counting kit-8 and quantitative polymerase chain reaction, respectively. Chondrocytes with high viability could be successfully isolated from fetal cartilage tissue and exhibited adherence to in vitro surfaces. The addition of transferrin and ethanolamine to low-serum Dulbecco’s modified eagle medium/F12 culture medium promoted the expression of chondrocyte marker genes and cell proliferation. The supplementation of transferrin and ethanolamine in low-serum Dulbecco’s modified eagle medium/F12 culture medium was advantageous for enhancing the proliferation of fetal chondrocytes and suppressing dedifferentiation during cultivation.
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