Abstract

The current study was aimed to investigate the immunomodulatory and anti hyperglycemic activity of cow urine using Wistar albino rats as experimental animal model. Three doses of cow urine (2, 4 and 6 ml/kg body weight) were administered to rats and selected on the basis of random sampling method. Neutrophil adhesion and delayed hypersensitivity tests were done to assess the cellular immunity, whereas humoral immunity was investigated by indirect haemagglutination titer assay. Diabetes was induced in rats by the administration of alloxan (150 mg/kg, intraperitoneal). The effect of different doses of cow urine and Glibenclamide (standard drug) was observed for consecutive 3 w. At dose 4 and 6 ml/kg, a significant increase in neutrophil adhesion and delayed type hypersensitivity responses were observed. The circulating antibody titer was significantly increased in animals treated with 6 ml/kg. Surprisingly, cow urine resulted in the significant decline in blood sugar level of diabetic animals as compared to diabetic control group. This shows that Badri cow urine has potential to modulate immune system via cellular and humoral immune responses. The results also indicate the dose-dependent antidiabetic activity of Badri cow urine. Hence, it may be used as alternative medicine in the treatment of diabetes and other related manifestations