Abstract
HSA_Circ_0134111 Regulates Chondrocyte Degeneration in Osteoarthritis by miR-103a/Smad7 Axis
Department of Orthopaedic Surgery, Shanghai Fifth People’s Hospital Affiliated to Fudan University, Minhang, Shanghai 200240, 1Department of Orthopaedic Surgery, Jinshan Branch Hospital of the Sixth People's Hospital of Shanghai, Jinshan, Shanghai 201599, China
Correspondence Address:
Minghai Wang, Department of Orthopaedic Surgery, Shanghai Fifth People’s Hospital Affiliated to Fudan University, Minhang, Shanghai 200240, China, E-mail: sking1972@163.com
To explore the mechanism of hsa_circ_0134111 regulating micro RNA-103a/Smad7 axis in chondrocyte degeneration in osteoarthritis. Human chondrocytes (HUM-CO-013) were cultured in vitro and divided into si-control group and si-circ_0134111 group. Chondrocytes were induced by monoiodoacetate to establish osteoarthritis injury model, the apoptosis rate was detected by flow cytometry, the expression of micro RNA-103a and Smad7 messenger RNA in osteoarthritis chondrocytes were detected by quantitative reverse transcription polymerase chain reaction; the targeting relationship between hsa_circ_0134111 and micro RNA-103a was detected by double luciferase report test. The contents of malondialdehyde, L-lactate dehydrogenase and glutathione peroxidase were detected by kit and the expressions of apoptosis-related proteins (B-cell lymphoma 2, B-cell lymphoma 2-associated protein x), collagen II, matrix metalloproteinase 13 and Smad7 in osteoarthritis chondrocytes were detected by Western Blot method. The apoptosis rate of osteoarthritis chondrocytes in si-circ_0134111 group was reduced than si-control group and the micro RNA-103a and Smad7 messenger RNA in osteoarthritis chondrocytes in si-circ_0134111 group were decreased than si-control group. Micro RNA-103a could reduce the activity of wild-type circ0134111 luciferase reporter gene, but had no effect on MUT-circ0134111. The contents of malondialdehyde and L-lactate dehydrogenase in osteoarthritis chondrocytes in si-circ_0134111 group decreased and the content of glutathione peroxidase increased, while the contents of B-cell lymphoma 2 and collagen II in osteoarthritis chondrocytes in si-circ_0134111 group increased, while the contents of B-cell lymphoma 2-associated protein x, matrix metalloproteinase 13 and Smad7 decreased compared with si-control group. Hsa_circ_0134111 can participate in osteoarthritis chondrocyte degeneration through micro RNA-103a/Smad7 axis and has_circ_0134111/micro RNA-103a/Smad7 axis may be a new regulatory mechanism and therapeutic target for osteoarthritis.
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