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Abstract
HPTLC Method Development For Pharmacokinetic Study Of Sparflaxacin In Plasma
Author(s): R. R Shah, B. N Suhagia, I. S Rathod, S. A Shah, D. M Patel
A simple, sensitive, specific and precise method has been developed for the analysis of the sparfloxacin in plasma. Extraction of sparfloxacin from plasma was done with dichloromethane. Standard solutions and extracted samples were spotted on precoated silica gel G 60 F 254 plates and developed using chloroform:toluene:methanol:diethylamine (4:4:1.5:0.2 v/v) as mobile phase. Densitometric scanning was performed at 301 nm. The method had linearity range of 80-200 ng/spot and average recovery was found to be 89.17 %.This method has been successfully used for the estimation of sparfloxacin in plasma samples of volunteers. The pharmacokinetic parameters like Cmax, Tmax, and AUC0-α were computed.
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