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Abstract
HPLC method for estimation of metformin hydrochloride in formulated microspheres and tablet dosage form
Author(s): Mousumi Kar, PK Choudhury
Department of Pharmaceutical Sciences, Mohan Lal Sukhadia University, Udaipur-313 001, India
Correspondence Address:
Mousumi Kar Department of Pharmaceutical Sciences, Mohan Lal Sukhadia University, Udaipur-313 001 India karmousumi@hotmail.com
Department of Pharmaceutical Sciences, Mohan Lal Sukhadia University, Udaipur-313 001, India
Correspondence Address:
Mousumi Kar Department of Pharmaceutical Sciences, Mohan Lal Sukhadia University, Udaipur-313 001 India karmousumi@hotmail.com
A simple, accurate, economical and reproducible HPLC method has been developed for quantitative estimation of metformin hydrochloride from tablet dosage form and formulated microspheres. The developed HPLC method is a reverse phase chromatographic method using phenomenex C 18 column and acetonitrile:phosphate buffer (65:35) pH adjusted to 5.75 with o-phosphoric acid as mobile phase and glipizide as internal standard. The linearity was observed in concentration range of 0-25 μg/ml for metformin hydrochloride. Results of analysis were validated statistically and by recovery studies.
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