Abstract
High Performance Liquid Chromatography-Tandem Mass Spectrometry for the Determination of Bile Acids in Mouse Serum
Department of Pharmacy, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250016, Department of Pharmacy, the Second Hospital of Shandong University, Jinan 250033, Department of Endocrinology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250016, China
Correspondence Address:
Department of Pharmacy, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250016, China E-mail: ttdoccn@126.com
This study is aimed to develop and validate a high-performance liquid chromatography-tandem mass spectrometry method for the determination of bile acids in mouse serum. The serum samples were analysed after solid-phase extraction. The analytes were separated on a Diamonsil C18 column with a mobile phase of methanol and water containing 10 mM ammonium acetate and 0.005% formic acid (70:30) at a flow rate of 0.5 ml/min. The analytes were detected by tandem mass spectrometry in negative ion mode. The results demonstrated that the calibration curve was linear for all bile acids over a range of 10~10 000 μg/l. Specificity, matrix effect, recovery, linearity, accuracy and precision were validated for bile acids in mouse serum. The high-performance liquid chromatography-tandem mass spectrometry method was found to be selective, sensitive, simple and was successfully applied to determine the bile acids in more than 200 mouse serum samples. In conclusion, this method is suitable for the quantitative detection of bile acids.
