Abstract

The present study investigates the expression of long non-coding RNA hypoxia-inducible factor-1 alpha-antisense 1 in albino rats with type 2 diabetes mellitus and its correlation with bone mineral density and bone metabolism markers. The research included comparing type 2 diabetes mellitus albino rats with healthy controls. The diabetic model was confirmed by significant weight loss and elevated blood glucose levels after a high-fat diet and streptozotocin injection. Serum analysis showed that diabetic rats had significantly lower calcium levels and higher alkaline phosphatase levels compared to controls. Bone mineral density was measured using dual-energy X-ray absorptiometry before and after ovariectomy in two groups of rats. Post-ovariectomy, control group exhibited a significant reduction in bone mineral density, confirming the establishment of an osteoporosis model. Reverse transcription polymerase chain reaction analysis revealed that long non-coding RNA hypoxia-inducible factor-1 alpha-antisense 1 expression was significantly lower in diabetic rats compared to controls. These findings suggest that long non-coding RNA hypoxia-inducible factor-1 alpha-antisense 1 is inhibited in diabetic osteoporotic rats and may play a role in the bone metabolism alterations observed in type 2 diabetes mellitus.