Abstract

Role of the Mucin 1 gene in thyroid cancer and its relationship with the adenosine-monophosphate activated-protein kinase-mammalian target of rapamycin signaling pathway were investigated in this work. The ACT-1 thyroid cancer cell lines were employed as the research models and enrolled into three groups based on treatment viz., a control group (no intervention), a small interfering ribonucleic acid-negative control group (transfection of negative control small interfering ribonucleic acid at 100 nM/l into ACT-1 thyroid cancer cells), and a Mucin 1-small interfering ribonucleic acid group (transfection of Mucin 1-small interfering ribonucleic acid at 100 nM/l into ACT-1 cells). The impacts of interfering with Mucin 1 gene on thyroid cancer cell proliferation, apoptosis, adenosine-monophosphate activated-protein kinase, mammalian target of rapamycin, and autophagy-related proteins (light chain 3I and light chain 3 II) were observed. The results indicated that the cell survival rate in Mucin 1-small interfering ribonucleic acid group (53.15±8.23) % was lower to that in control group (95.13±6.42) % (p<0.05), resulting a higher cell apoptosis rate of (15.79±1.36) %, which was (3.11±0.68) % in control group (p<0.05). p-Mammalian target of rapamycin in control group (0.13±0.02) was elevated based on that in Mucin 1-small interfering ribonucleic acid group (0.05±0.01) (p<0.05), while p-adenosine-monophosphate activated-protein kinase in control group (0.16±0.02) was downshifted in contrast to that in Mucin 1-small interfering ribonucleic acid group (0.46±0.03) (p<0.05). Light chain 3I/light chain 3II in control group was (0.28±0.02), being lower in comparison to that in Mucin 1-small interfering ribonucleic acid group (0.46±0.03) (p<0.05). Interference with Mucin 1 gene regulated the biological behaviors of thyroid cancer cells by modulating the adenosine-monophosphate activated-protein kinase-mammalian target of rapamycin signaling pathway.