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Abstract
Estimation Of Valdecoxib In Tablets By RP-HPLC Method
Author(s): A Suganthi, H. B Sivakumar, Spana Shrikumar, M Gandhimathi, M Gopala Rao, T. K Ravi
A simple, efficient and reproducible reverse phase HPLC method has been developed for the determination of valdecoxib in tablets. The analyte was resolved by using a mobile phase (water:acetonitrile 50:50) at the flow rate of 1.2 ml/min on an isocratic HPLC system (Shimadzu) consisting of LC 10AT liquid pump, SPD 10 A UV/V is detector, Shimadzu class LC 10 A software and Water's Spherisorb ODS column (0.4x150 mm, 0.5 μ particle size) at a wave length of 237 nm. An external standard calibration method was employed for quantitation. The linearity range was 0.2-5 μg/ml for valdecoxib and the elution time was 2.4 min. The percentage recovery obtained for two brands were 100.2±0.22 and 99.6±0.24, respectively.
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