Abstract

Numerous papers stated that Marsdenia tenacissima extract possessed tumor-suppression properties. Herein, we aimed to investigate the influence and underlying mechanism of Marsdenia tenacissima extract on oral squamous cell carcinoma. After being cultured, CAL-27 cells were treated with various doses of Marsdenia tenacissima extract. Proliferation, apoptosis, and migration were assessed using cell counting kit-8, clone formation, scratch, and flow cytometry assays. Western blot detected protein levels. Quantitative reverse transcription polymerase chain reaction method analyzed hsa_circular_0003645 and microRNA-335 expression. Their interaction was validated using dual-luciferase reporter gene experiment. 1.0 and 1.5 mg/ml Marsdenia tenacissima extract or si-hsa_circular_0003645 could suppress CAL-27 cell proliferation, migration, N-cadherin, and hsa_circ_0003645, whereas increase apoptosis, E-cadherin, and microRNA-335. Furthermore, the upregulation of hsa_circular_0003645 might abolish Marsdenia tenacissima extract exposure-mediated oral squamous cell carcinoma cell proliferation and migration inhibition and apoptosis promotion. Besides, hsa_circular_0003645 could target microRNA-335. Marsdenia tenacissima extract treatment might hinder oral squamous cell carcinoma progression via regulating hsa_circular_0003645 and microRNA-335.