Abstract
Effects of Zerumbone Combined with Paclitaxel on Molecules Related to Immunogenic Cell Death of Human Salivary Gland Mucoepidermoid Carcinoma Cells
Department of Stomatology, Southwest Medical University, Luzhou, Sichuan Province 626000, 1Department of Stomatology, 2Department of Medicine, Suining Central Hospital, Suining, Sichuan Province 629000, People’s Republic of China
Correspondence Address:
Sen Yang, Department of Stomatology, Suining Central Hospital, Suining, Sichuan Province 629000, People’s Republic of China, E-mail: ys13880435413@163.com
To investigate the effects of zerumbone and paclitaxel on immunogenic cell death in human salivary gland mucoepidermoid carcinoma MC-3 cell line. The MC-3 cell line of mucoepidermoid carcinoma was selected for culturing, and the viability of MC-3 was detected by the cell counting kit-8 method, and the concentration of zerumbone was screened for the half inhibition rate of zerumbone in MC-3 cell line at half-maximal inhibitory concentration. The apoptosis rate was detected by flow apoptosis method; calreticulin and transmembrane glycoprotein cluster of differentiation 47 were detected by immunofluorescence method and flow cytometry. High mobility group protein B1 was detected by in situ hybridization. Zerumbone acted for 48 h at a concentration of 40 µmol/l to reach half-maximal inhibitory concentration (p<0.01). Zerumbone promoted MC-3 apoptosis as well as inhibited MC-3 proliferation and migration, and its effect was enhanced when combined with paclitaxel (p<0.05). Zerumbone induced an increase in the expression of calreticulin and high mobility group box 1 in the molecules associated with MC-3 immunogenic death (p<0.01), and the adenosine triphosphate content was elevation (p<0.05), and enhanced its effect when combined with paclitaxel (p<0.05). Zerumbone induced attenuation of cluster of differentiation 47 expression in MC-3 immunogenic death-related molecules (p<0.05), and the attenuation effect was not enhanced when combined with paclitaxel. Zerumbone can promote MC-3 apoptosis and inhibit its proliferation and migration, and its effects are enhanced in combination with paclitaxel. Zerumbone can induce immunogenic cell death in salivary gland mucoepidermoid carcinoma cells by affecting the levels of damage-related molecules, calreticulin, cluster of differentiation 47, adenosine triphosphate, and high mobility group box 1, and the ability of flower gingerone and paclitaxel in salivary gland mucoepidermoid carcinoma lines to induce immunogenic cell death was enhanced when the combination of both was administered.
Full-Text | PDF
