Abstract

To exam the effects of transforming growth factor-beta1/suppressor of mothers against decapentaplegic signal mediated by poly L-lactic acid on biological behavior and collagen expression of dermal fibroblasts. Dermal fibroblasts Hs60 were treated with different concentrations of poly L-lactic acid. Quantitative polymerase chain reaction was used to detect the collagen type I alpha 1, collagen type I alpha 2, elastin, matrix metalloproteinases-1, tissue inhibitors of metalloproteinases-1 and tissue inhibitors of metalloproteinases-2, messenger ribonucleic acid level; procollagen synthesis was analyzed using a type I prolactin inducible protein enzyme immunoassay kit, and the amount of proteins associated to the transforming growth factorbeta 1/suppressor of mothers against decapentaplegic signal pathway was measured using Western blot. The procollagen products in the high poly L-lactic acid intervention group (group C) was raised than that in the low- poly L-lactic acid intervention group (group B) and the control group (group A). The collagen type I alpha 1 and collagen type I alpha 2 protein in the group C were increased than those in the lowpoly L-lactic acid intervention group and the group A. The elastin, metalloproteinases-1, tissue inhibitors of metalloproteinases-1 and tissue inhibitors of metalloproteinases-2 messenger ribonucleic acid in group C was raised than that in low-poly L-lactic acid intervention group and group A, while the metalloproteinases-1, messenger ribonucleic acid in group B was reduced than that in group C and group A. The transforming growth factor-beta1 and suppressor of mothers against decapentaplegic messenger ribonucleic acid in group C was raised than that in low-poly L-lactic acid intervention group and group A. The transforming growth factor-beta1 and suppressor of mothers against decapentaplegic protein in the group C were increased than those in the low-poly L-lactic acid intervention group and the group A. Poly L-lactic acid stimulates collagen expression and synthesis in dermal fibroblasts by activating transforming growth factor-beta1/suppressor of mothers against decapentaplegic signal pathway.