Abstract

The effect of recombinant human bone morphogenetic protein-2 mediated wingless-related integration site beta-catenin pathway on bone defect repair and angiogenesis of rabbit radius was explored. 18 New Zealand rabbits were divided into 3 groups, control group (group A), low dose recombinant human bone morphogenetic protein-2 group (group B), and high dose recombinant human bone morphogenetic protein-2 group (group C). The bone mineral density of each group was compared. Rabbit bone marrow mesenchymal stem cell line cells were resuscitated and the proliferation ability, protein expression, messenger ribonucleic acid expression and cell migration ability of the three groups were detected by cell counting kit-8, Western blot, quantitative polymerase chain reaction and Transwell methods. The radial bone mineral density of rabbits in the group B was higher than that in group A at 4 w and 8 w, while in group C was higher than that group B. The cell proliferation and migration activity in the group B increased than group A, while those in the group C were raised than the group B. The protein levels of angiopoietin-1and vascular endothelial growth factor in group B increased than those in the group A and these levels in group C raised comparatively than in group B. The relative expression of wingless-related integration site beta and catenin in group B reduced than group A, while this expression in group C was lower than group B. Recombinant human bone morphogenetic protein-2 can promote the repair of radial bone defect and angiogenesis by mediating wingless-related integration beta-catenin pathway in rabbits.