Abstract

This study was to reveal the effect of rehabilitation nursing training on nerve repair in rats with cerebral ischemic stroke as well as the effect of rehabilitation nursing training on brain protection and its potential molecular mechanism. It was also aimed to provide objective experimental basis for the implementation of clinical nursing measures. Adult male Sprague Dawley rats were randomly divided into 3 groups, the sham-operated group, non-exercise group and early-exercise group. On this basis, a rat model of middle cerebral artery occlusion was established. After 24 h of cerebral ischemia and perfusion, the rats were subjected to treadmill nursing training intervention for 30 min once a day. Finally, the neuronal apoptosis and the expression of MMP-9 and MMP-2 in rats were observed by labelled staining protein and the mechanism of rehabilitation nursing training on the repair and protection of neuronal function in rats was further discussed. The results showed that there was no significant change in neurological deficits in the sham-operated group, but neurological deficits in the middle cerebral artery occluded rats were significantly impaired. There was a significant difference between the sham-operated group and the nursing training group (p<0.01). In the ischemic control group, the recovery rate of nerve function after ischemic injury was slow, and even the neurological dysfunction was aggravated. It was most severe on the day 3 and would recover slowly thereafter. After cerebral ischemia injury, the expression of MMP-2 protein began to increase gradually until the d 3 after operation. The expression of MMP-2 in the sham-operated group remained at a low level, while the expression of MMP-2 in the ischemic control group was significantly higher than that in nursing training group. The differences between the nursing training group and the sham-operated group and the nursing training group and the ischemic control group were statistically significant (p<0.01). It can be concluded that stroke rehabilitation nursing training could improve the recovery effect of neurological function and protect brain tissue, and its mechanism might be related to inhibiting signal transduction pathway and regulating the expression of MMP-9 and MMP-2 protein.