Abstract

To investigate the diagnostic value of chitinase-3-like protein 1 and keratin 5 in bronchoalveolar lavage fluid in 88 patients with lung disease. This was a case control study to collect 50 patients with lung cancer (malignant disease group) and 38 patients with benign lung disease (benign disease group) who were admitted to the respiratory medicine department in the affiliated hospital of medical school of Ningbo university from December 2019 to March 2020. The enzyme-linked immunosorbent assay was applied to detect the expression levels of chitinase-3-like protein 1 and keratin 5 in bronchoalveolar lavage fluid of the two groups of the patients. The diagnostic effect was evaluated using the subject's working characteristics to establish a diagnostic mathematical model. The levels of chitinase-3-like protein 1 and keratin 5 in bronchoalveolar lavage fluid were significantly higher in the malignant disease group than in the benign disease group (p<0.05). These two (chitinase-3-like protein 1 and keratin 5) were significantly correlated and positively correlated and the combined test could significantly improve the sensitivity and specificity of lung cancer diagnosis. The diagnostic rate of chitinase-3-like protein 1 and keratin 5 for lung cancer. The chitinase-3-like protein 1 in bronchoalveolar lavage fluid and carcinoembryonic antigen in blood combined was superior to chitinase-3- like protein 1 or keratin 5 alone in the diagnosis of lung cancer. Chitinase-3-like protein 1 and keratin 5 in bronchoalveolar lavage fluid contribute to the early diagnosis of lung cancer and can be used as biomarkers for the diagnosis of lung cancer with high sensitivity and specificity. The application of the two combined assays as molecular markers for clinical diagnosis and as targeted therapeutic agents needs to be further studied.