Abstract

A new reversed-phase high performance liquid chromatography method was developed and validated for the simultaneous determination of losartan potassium and atenolol in tablets. The separation was achieved on Supelcosil ODS analytical column (25×0.46 cm, i.d., 5 µm) using acetonitrile and 25 mM potassium dihydrogen phosphate (45:55 v/v, pH 3.00±0.05) as mobile phase at a flow rate of 1.2 ml/min. Detection was carried out using a UV detector at 227 nm. The method was validated. The developed and validated method was successfully applied for the quantitative analysis of Losar beta® tablets. The total chromatographic analysis time per sample was about 6 min with atenolol, chlorzoxazone (internal standard) and losartan eluting at retention times of about 2.72, 4.89 and 5.61 min, respectively. The standard curves were linear over the concentration ranges, 1 to 10 µg/ml for losartan potassium and atenolol. The values obtained of LODs were 0.029 and 0.062 µg/ml and LOQs were 0.078 and 0.187 µg/ml for losartan potassium and atenolol, respectively. The proposed method is fast, accurate and precise for the determination of losartan potassium and atenolol for routine quality control of tablets containing these two drugs.