Abstract

Herbs are the main source of polyherbal formulations. The potency of polyherbal formulations depends on their ingredients. The evaluation parameters for polyherbal formulations are based on chemical, physical and microbiological assessment. The aim of the present study was to formulate and standardize Palash beejadi churna and develop a simple, accurate and reproducible high performance thin layer chromatography method for simultaneous estimation of phytoconstituents in the formulation. Embelin and conessine were selected as the markers for development of high performance thin layer chromatography method. The chromatographic analysis was performed on pre-coated thin layer chromatography plates with silica gel G60 F254 using toluene:ethyl acetate:formic acid:ammonia:diethyl amine (3:3:1.5:0.5:2.5 v/v/v/v/v) as solvent system. Both the markers were scan at 520 nm. The retention factors values of conessine and embelin were 0.46 and 0.87, respectively. Linearity for conessine and embelin was observed in the ranges 2000-6000 ng/spot. Limit of detection and limit of quantification values of conessine and embelin were found to be 624.5 and 768.5, 1892 and 2328 (ng/spot), respectively. The average percent recovery of conessine and embelin was found to be 98 % and 101 %, respectively. The SD and percent relative SD for of intraday and interday precision of both the markers were found to be <2 % respectively. The high performance thin layer chromatography method was validated as per the International Council for Harmonisation guideline and found to be simple, precise, specific, sensitive and accurate that can be used for routine quality control of raw material as well as formulation containing any of these markers.