Abstract

A simple, accurate, precise and economic Ultraviolet spectrophotometric method has been developed for the simultaneous estimation of amlodipine, perindopril and indapamide. The developed method was based on determination of the three drugs using Ultraviolet absorbance correction method. The three wavelengths chosen were 365 nm for amlodipine as absorbances due to other two drugs were nil at this wavelength, 245 nm for indapamide (corrected for absorbance due to amlodipine) and 204 nm for perindopril (corrected for absorbances due to amlodipine and indapamide) with water as diluent. The Beer Lambert’s range for the three drugs was 10-60 μg/ml, 5-20 μg/ml and 10-100 μg/ml for amlodipine, indapamide and perindopril respectively, with correlation coefficient (r2) of ≥0.999. The developed method was validated as per International Conference on Harmonisation guidelines and was found to be accurate, precise, sensitive and robust. The percentage assay results were within acceptable limits. Hence the developed method can be successfully used for the routine analysis of amlodipine, perindopril and indapamide in bulk and in combination.