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Abstract
Determination of 1,7,7-trimethyl-bicyclo(2,2,1)heptan-2-one in a cream pharmaceutical formulation by reversed-phase liquid chromatography
Author(s): GA Shabir, TK Bradshaw
Oxford Brookes University, School of Life Sciences, Oxford, OX3 0BP, United Kingdom
Correspondence Address:
G A Shabir Oxford Brookes University, School of Life Sciences, Oxford, OX3 0BP United Kingdom E-mail: gshabir.oxford@yahoo.com
Oxford Brookes University, School of Life Sciences, Oxford, OX3 0BP, United Kingdom
Correspondence Address:
G A Shabir Oxford Brookes University, School of Life Sciences, Oxford, OX3 0BP United Kingdom E-mail: gshabir.oxford@yahoo.com
A reversed-phase liquid chromatographic method for the determination of 1,7,7-trimethyl-bicyclo(2,2,1)heptan-2-one in a cream formulation is developed and validated. The separation was achieved using an isocratic mobile phase, on a Lichrosorb C8 column. The calibration curve is linear (r 2 = 0.9999) from 25-175% of the analytical concentration of 1.0 mg/ml. The mean percent standard deviation values for intra-day and inter-day precision studies were <1%. The recovery ranges 99.80-100.06% from a cream formulation. The method can be used reliably in quality control for the analysis of bulk cream samples and final product release.
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