Abstract

The antibiogram study of methicillin resistant Staphylococcus aureus isolates revealed 100% resistance to vancomycin, bacitracin, erythromycin, ciprofloxacin and nalidixic acid. Eight isolates (53.3%) showed resistance to co-trimoxazole and one isolate to rifampicin, which was the drug of choice. An effort was made to evaluate the antimethicillin resistant Staphylococcus aureus activity of silver oxide (Ag 2 O) nanoparticles synthesized from Aspergillus terreus VIT 2013. Production of Ag 2 O nanoparticles was confirmed by color change of fungal filtrate and UV light absorption at 450 nm. X-ray diffraction pattern showed 2θ values at 27, 32, 38 and 57°, which corresponded to the cubic structure of Ag 2 O nanocrystals. Fourier transform infrared spectroscopy indicated the presence of primary amine, carbonyl group, NO 2 and silver, revealing protein mediated nanoparticle production. The scanning electron microscope image showed freely dispersed Ag 2 O nanoparticles. The nanoparticles were active against all methicillin resistant isolates and hence can be used as antibacterial agents against drug resistant bacteria.