Abstract
Colorimetry method for estimation of glycine, alanine and isoleucine
1 Department of Quality Assurance, L. M. College of Pharmacy, Navrangpura, Ahmedabad - 380 009, India 2 Department of Pharmaceutical Chemistry, Shri Sarvajanik College of Pharmacy, Near Aravind Baug, Mehsana - 384 001, India
Correspondence Address:
S A Shah Department of Quality Assurance, L. M. College of Pharmacy, Navrangpura, Ahmedabad - 380 009 India E-mail: shrini_s@hotmail.com
A simple and sensitive colorimetry method has been developed for estimation of amino acids glycine, alanine and isoleucine. Amino acids were derivatized with dichlone in presence of sodium bicarbonate. Amino acids showed maximum absorbance at 470 nm. The method was validated in terms of linearity (5-25 µg/ml for glycine, alanine and isoleucine), precision (intra-day variation 0.13-0.78, 0.22-1.29, 0.58-2.52% and inter-day variation 0.52-2.49, 0.43-3.12, 0.58- 4.48% for glycine, alanine and isoleucine respectively), accuracy (91.43-98.86, 96.26-105.99 and 95.73-104.82 for glycine, alanine and isoleucine respectively), limit of detection (0.6, 1 and 1 µg/ml for glycine, alanine and isoleucine respectively) and limit of quantification (5 µg/ml for glycine, alanine and isoleucine). The method was found to be simple and sensitive.
