Abstract

Multidrug resistance is an emerging major clinical problem rapidly enhancing throughout the world, which require novel effective drugs to control this problem. The present study was aimed at investigation of silver nanoparticles synthesized by Micromonospora species for antibacterial activity against hospitalacquired uropathogens. Marine actinomycetes were collected from Kayalpatnam, located at Tuticorin, Tamil Nadu, India. Totally, six actinomycetes were isolated and identified based on their spore formation and biochemical studies. Six isolates were belonged to the genera of Micromonospora species. Among the six, Micromonospora species, KPMS10 showed potent antibacterial activity against Gram-negative and Gram-positive uropathogens. Silver nanoparticles synthesized by Micromonospora species KPMS10 were isolated, characterized using ultra violet-visible spectroscopy, Fourier transform infrared spectroscopy and scanning electron microscopy. The shape of nanoparticle was found to be spherical with an average size of 80 nm. The antibacterial activities of silver ion against uropathogens were found to be superior to third generation cephalosporins. These silver nanoparticles were found to be least haemolytic at 20 µg/ml. Biosynthesis of silver in combination with actinomycetes would be effective against clinical multidrug-resistant pathogens.