Abstract

The human red blood cell blood group system plays an extremely important role in blood transfusion medicine and the ABO blood group system is of great significance in clinical blood transfusion, organ transplantation, forensic medicine and genetics research. The aim of this study was to study the application of genotyping technology in difficult ABO blood types identification. A total of 12 patients were selected in our hospital from August 2019 to August 2020. Among them, 1 patient with peptic ulcer, 1 patient with chronic kidney disease stage V, 2 patients with diabetes, 1 patient with infertility, 3 patients with anemia, 1 patient with primary liver cancer, 2 patients with leukemia and 1 patient with systemic lupus erythematosus. Polymerase chain reaction-sequence specific primers genotyping method was used to detect these patients with difficult ABO blood type antigens and statistical method was used to analyze the correlation of ABO genotypes and difficult ABO blood types. The specific distribution of ABO genotypes in 12 patients were detected as follows: 6 cases of A/B, 3 cases of A/O and 3 cases of B/O. The genotype detected by polymerase chain reaction-sequence specific primers was consistent with serological phenotype. As an auxiliary method, ABO genotyping can be used to detect difficult ABO blood types.