Abstract
Application of Ratio Spectra Derivative Method for Simultaneous Estimation of Moxifloxacin Hydrochloride and Loteprednol Etabonate from Ophthalmic Formulation
Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Charusat Campus, Changa, Gujarat 388421, India
Correspondence Address:
Kanan Gamit, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Charusat Campus, Changa, Gujarat 388421, India, E-mail: kanangamit.ph@charusat.ac.in
A simple, precise and accurate ultraviolet-visible spectrophotometer method has been developed by applying ratio spectra derivative method and validated for the simultaneous estimation of moxifloxacin hydrochloride and loteprednol etabonate from ophthalmic formulation. Absorbance of moxifloxacin hydrochloride and loteprednol etabonate was recorded at their respective wavelength and then zero order spectra was converted to first order derivative spectra after dividing respective zero order spectra with divisor concentration. Developed method was linear at concentration range 4-20 µg/ml, (r2 =0.99) and (r2 =0.99), for loteprednol etabonate and moxifloxacin hydrochloride respectively. The linear regression equations were y=0.005x+0.001 for loteprednol etabonate and y=0.118x-0.062 for moxifloxacin hydrochloride. The results of analysis were validated which showed that proposed method was simple, precise and accurate.
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