Abstract
Amygdalin Inhibits Cell Proliferation, Migration and Invasion in Thyroid Cancer by Regulating CircWHSC1/miR-195-5p
Department of General surgery, Pingyang Hospital affiliated to Wenzhou Medical University, 1Department of Pharmaceutical, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, 2College of Life and Environmental Science, Wenzhou University, Whenzhou 325000, China
Correspondence Address:
Xiaozhen Zhu, Department of Pharmaceutical, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Whenzhou 325000, China, E-mail: ZXZ1632021@163.com
To probe the functions of amygdalin on thyroid cancer cells and further analyze whether its mechanism is related to regulating circWHSC1 and miR-195-5p expression. The relative levels of circWHSC1 and miR-195-5p in cells were analyzed by reverse transcription-quantitative polymerase chain reaction. Effects of amygdalin on thyroid cancer cell lines were calculated. Dual luciferase report assay was applied to ensure the targeting between circWHSC1 and miR-195-5p. The level of circWHSC1 was increased, while the level of miR-195-5p was declined in thyroid cancer tissues. Amygdalin treatment dose-dependently reduced cell viability, suppressed cell invasion, migration and colony formation abilities. Amygdalin treatment reduced the level of circWHSC1, as well as increased the level of miR-195-5p. CircWHSC1 directly bound to miR-195-5p. CircWHSC1 inhibition notably restrained cell growth and mobility in thyroid cancer. Moreover, circWHSC1 overexpression abated the suppressing functions of amygdalin on thyroid cancer cell proliferative, migratory and invasive abilities. Amygdalin inhibited cell proliferative, migratory and invasive abilities in thyroid cancer via circWHSC1/miR-195-5p axis.
